Introduction

Dengue igg igm rapid test device is a lateral flow chromatographic immunoassay for the simultaneous detection and differentiation of IgM and lgG anti- dengue in human serum (or plasma). The kit is a diagnostic device for qualitative detection

Summary

When a specimen is added to the test, lgG and lgM antibodies in the specimen sample react with gold particles labled withdengue envelope proteins. As this specimen/particle mixture migrates along the length of the test, the anti-dengue lgG and/or lgM antibody particle complex is captured by the relevant lgG and IgM test bands located in the test device window causing a pale to dark red band to form at the test region of the cassette window. T he intensity of the bands will vary depending upon the amount of antibody present in the sample. The appearance of any color in test region should be considered as positive for lgG and/or IgM

antibody. A red procedural control line should always develop in the test device window to indicate that the test has been performed properly.

Specimen Collection

For serum or plasma, collect blood into acontainer without anticoagulant. If the specimen cannot be tested on the day of collection, store the serum or plasma specimen in a refrigerator or freezer Bring the specimens to room temperature before testing.

Interpretation of Results

Negative:Only control pink band appears on test region of the cassette. This indicates that there is no anti-dengue lgM and lgG in the specimen.

Positive: Three pink bands(C G,M) ,two pink bands(C,G) or(C,M) appear on test region ofthe cassette. This indicates that the specimen contains detectable amount ofanti-dengue lgGand lgM,only lgG, only IgM.

Invalid: If without colored band appears at control region, this is an indication of a possible error in performing the test. The test should be repeated using a new one.

Dengue test is a rapid immunochromato graphicassay for the simultaneous detection of lgG and IgM antibodies to dengue virus in human whole blood, serum or plasma. The assay is used as a screening test for Dengue viral infection and as an aid for differential diagnosis of primary and secondary infections in conjunction with other criteria.